Characterizing AAV Charge Heterogeneity Using High-Resolution icIEF Fractionation with LC-MS Analysis

Adeno-associated viral (AAV) gene therapy products, which contain a DNA transgene packaged into a protein capsid, have shown tremendous therapeutic potential in recent years for a range of diseases. Technologies like imaged capillary isoelectric focusing (icIEF) are designed to help scientists make these products a reality by providing an orthogonal, fast, and high-resolution method for AAV charge species collection and characterization. Addressing charge heterogeneity is crucial as it can affect the stability and biodistribution of AAV-based biotherapeutics.

In this GEN webinar, our speakers will discuss icIEF technology and its application to protein charge analysis in AAV gene therapy development. During the webinar, you’ll learn about the MauriceFlex icIEF system, a protein fractionation solution the enables fraction collection for charge variant analysis and eliminates the need for mobilization after separation while providing data in 10-15 minutes. You’ll also hear about recent innovations that enable the direct characterization of charge isoforms by icIEF separation by mass spectrometry (MS) including a novel methodology for AAV viral protein species separation by icIEF and characterization by MS.

Key learnings for this webinar include:

• How the protein fractionation capabilities of MauriceFlex enable fraction collection for charge variant analysis
• A methodology for AAV viral protein species separation by icIEF and characterization by MS
• The advantages of using icIEF fractionation over traditional ion-exchange chromatography (IEX)-based fractionation

A live Q&A session will follow the presentation, offering you a chance to pose questions to our expert panelist.