Rapid Synthesis of Full-Length High-Complexity Clonal Genes

Constructing plasmids containing complex full-length genes can be cumbersome. The iterative construction process, which involves stitching smaller gene fragments together, is longer and more error-prone for complex structural elements, such as inverted terminal repeats or long repeats. Errors in the initial building blocks or the construction process accumulate, necessitating the screening of multiple colonies for each construct to identify a sequence-perfect plasmid.

In this GEN webinar, Elegen’s Galit Meshulam-Simon, PhD, and Matt Deaner, PhD, will discuss recent innovations in DNA synthesis that enable fast, accurate synthesis of long, complex plasmids. They will also share data that compares novel and conventional approaches. During the webinar, you will learn about Elegen’s proprietary cell-free cloning technology for highly accurate fragments for plasmid construction, and how it is used for building and expressing long and complex pathways such as the carotenoid biosynthesis pathway and additional reporter genes. Key takeaways from the webinar include:

• Robust ENFINIA^TM Plasmid DNA process

• The challenges of building long and complex biosynthetic pathways with conventional gene synthesis methods

• Case studies that highlight the benefits of switching from small error-prone fragments to long, accurate linear DNA to build large complex constructs

A live Q&A session will follow the presentation, offering you a chance to pose questions to our expert panelists.