Effective mAb Aggregate Removal: Evaluating Three Key Chromatography Resins

Producing novel antibody variants, including multi-specifics and even some traditional monoclonal antibodies (mAbs), can result in high levels of product-related impurities, including aggregates. These high molecular weight species (HMWS) must be reduced during downstream processing to ensure the safety and efficacy of therapeutic products. Traditionally, cation exchange chromatography has been the go-to method for removing HMWS. However, as impurity levels increase, alternative methods such as hydrophobic interaction and mixed-mode chromatography are emerging that are more selective.

In this GEN webinar, Alejandro Becerra, PhD, will present results from using an IgG1 post-Protein A pool as a model system to evaluate the aggregate removal capabilities of three Thermo Fisher chromatography resins under typical operating conditions. These results will highlight the variety of options that are available to manufacturers for mAb aggregate removal, the requirements for each resin type, and important considerations for implementing these tools in downstream processing. Key learnings from the webinar will include:

• The benefits of cation exchange, hydrophobic interaction, and mixed-mode chromatography resins for mAb purification
• Comparison of removal capabilities of POROS XS, POROS Benzyl Ultra, and POROS Caprylate chromatography resins
• Strategies for reducing HMWS levels across different column loadings, pH, and salt concentrations
• Guidelines for selecting the right chromatography resin for mAb aggregate removal

Pharmaceutical Grade Reagent. For Manufacturing and Laboratory Use Only.

A live Q&A session will follow the presentation, offering you a chance to pose questions to our expert panelist.