GenScript and Avectas agreed to partner to develop an improved non-viral cell therapy manufacturing process. By combining Avectas’ cell engineering technology with GenScript’s expertise in synthetic long oligo production, the partnership aims to demonstrate a novel and efficient solution for cell therapy manufacturing and to improve editing efficiency and cell viability over traditional delivery methods.

CRISPR-based non-viral gene editing methods have gained popularity among research teams following concerns about the FDA’s recent draft guidance on the use of viruses for gene and cell therapy. GenScript is collaborating with both academic and industry partners in the development of CRISPR non-viral gene editing to enable next-generation gene- and cell-therapy R&D projects.

Intracellular delivery is integral to the generation of engineered ex vivo cell-based therapies, including genome editing approaches. But the limitations of current delivery modalities, both viral and non-viral, led Avectas to develop the non-viral SOLUPORE® cell engineering technology, which enables efficient delivery of cargoes into cells for the development of next-generation therapies, explained Michael Maguire, PhD, CEO of Avectas.

SOLUPORE technology is a patented non-viral, cell engineering technology that permeabilizes the target cell membrane and allows transfer of cargo.

The research teams will apply SOLUPORE technology to permeabilize the target cell membrane so that engineered cargoes can be delivered while retaining high levels of cell viability and functionality. GenCRISPR™ synthetic sgRNA and Cas9 protein are then complexed into a ribonucleic protein that is co-delivered with GenExact™ ssDNA HDR templates into the cell nucleus.

“GenScript is excited to partner with Avectas as part of our program to develop novel RNP [ribonucleoproteins] and oligo delivery systems for non-viral cell engineering,” said Ray Chen, PhD, president of GenScript USA Life Science Group. “We expect this method will provide our customers with more complete solutions for efficient gene editing using our GenCRISPR sgRNA and ss/dsDNA HDR templates.”

“Avectas [looks forward to working ] with GenScript to combine its innovative editing tools together with our SOLUPORE delivery platform,” added Maguire. “This will enable the development of next-generation cell therapies differentiated by the quality of the modified cells, which retain high viability, functionality, and post-process proliferation.”

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